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human colonic carcinoma t84 cells  (ATCC)
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ATCC human colonic carcinoma t84 cells
Figure 5. Scavenger Receptors Are Involved in PorBIA-Mediated Invasion (A–C) Chang cells transfected with siGp96-1, siGp96-2, or siLuc were incubated with either 75 mg/ml Fucoidin, 10 mg/ml AcLDL, or no in- hibitor for 30 min. Then, cells were infected with N1135 (N927-GFP) in the absence of phosphate for 2 hr with an MOI of 100. (C) Ad- herent and invasive bacteria were stained dif- ferentially. Gonococci express GFP protein. Additionally, adherent bacteria were stained with polyclonal rabbit anti-gonococcal antise- rum and Cy3-conjugated goat, anti-rabbit IgG. In the overlay, adherent bacteria appear yellow, and invasive bacteria appear green. Adherent (A) and invasive (B) bacteria were quantified by gentamicin survival assays. Data represent the mean ± SEM of three inde- pendent experiments. (D) The Scavenger Receptor, SREC, is ex- pressed on epithelial cells. Real-time PCR was performed to determine the levels of the scavenger receptors, SR-AI, CD36, and SREC on HeLa, Chang, CHO cells as well as on differentiated and nondifferentiated phago- cytes (JOSK-M cells). Expression levels were normalized to the expression level on CHO cells. Data represent the mean ± SEM. (E) SREC is expressed in nontransformed cells. Real-time PCR was performed as described under (D) on END1 (cervix epithelial cells), <t>T84</t> (colon epithelial cells), and HUVEC (endo- thelial cells) mRNAs. Data represent the mean ± SEM.
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Figure 5. Scavenger Receptors Are Involved in PorBIA-Mediated Invasion (A–C) Chang cells transfected with siGp96-1, siGp96-2, or siLuc were incubated with either 75 mg/ml Fucoidin, 10 mg/ml AcLDL, or no in- hibitor for 30 min. Then, cells were infected with N1135 (N927-GFP) in the absence of phosphate for 2 hr with an MOI of 100. (C) Ad- herent and invasive bacteria were stained dif- ferentially. Gonococci express GFP protein. Additionally, adherent bacteria were stained with polyclonal rabbit anti-gonococcal antise- rum and Cy3-conjugated goat, anti-rabbit IgG. In the overlay, adherent bacteria appear yellow, and invasive bacteria appear green. Adherent (A) and invasive (B) bacteria were quantified by gentamicin survival assays. Data represent the mean ± SEM of three inde- pendent experiments. (D) The Scavenger Receptor, SREC, is ex- pressed on epithelial cells. Real-time PCR was performed to determine the levels of the scavenger receptors, SR-AI, CD36, and SREC on HeLa, Chang, CHO cells as well as on differentiated and nondifferentiated phago- cytes (JOSK-M cells). Expression levels were normalized to the expression level on CHO cells. Data represent the mean ± SEM. (E) SREC is expressed in nontransformed cells. Real-time PCR was performed as described under (D) on END1 (cervix epithelial cells), T84 (colon epithelial cells), and HUVEC (endo- thelial cells) mRNAs. Data represent the mean ± SEM.

Journal: Cell host & microbe

Article Title: Host glycoprotein Gp96 and scavenger receptor SREC interact with PorB of disseminating Neisseria gonorrhoeae in an epithelial invasion pathway.

doi: 10.1016/j.chom.2007.11.002

Figure Lengend Snippet: Figure 5. Scavenger Receptors Are Involved in PorBIA-Mediated Invasion (A–C) Chang cells transfected with siGp96-1, siGp96-2, or siLuc were incubated with either 75 mg/ml Fucoidin, 10 mg/ml AcLDL, or no in- hibitor for 30 min. Then, cells were infected with N1135 (N927-GFP) in the absence of phosphate for 2 hr with an MOI of 100. (C) Ad- herent and invasive bacteria were stained dif- ferentially. Gonococci express GFP protein. Additionally, adherent bacteria were stained with polyclonal rabbit anti-gonococcal antise- rum and Cy3-conjugated goat, anti-rabbit IgG. In the overlay, adherent bacteria appear yellow, and invasive bacteria appear green. Adherent (A) and invasive (B) bacteria were quantified by gentamicin survival assays. Data represent the mean ± SEM of three inde- pendent experiments. (D) The Scavenger Receptor, SREC, is ex- pressed on epithelial cells. Real-time PCR was performed to determine the levels of the scavenger receptors, SR-AI, CD36, and SREC on HeLa, Chang, CHO cells as well as on differentiated and nondifferentiated phago- cytes (JOSK-M cells). Expression levels were normalized to the expression level on CHO cells. Data represent the mean ± SEM. (E) SREC is expressed in nontransformed cells. Real-time PCR was performed as described under (D) on END1 (cervix epithelial cells), T84 (colon epithelial cells), and HUVEC (endo- thelial cells) mRNAs. Data represent the mean ± SEM.

Article Snippet: Cell lines used in this study included Chang human conjunctiva cells (ATCC CCL20.2); HeLa human cervix carcinoma cells (ATCC CCL2); CHO-K1 chinese hamster ovary cells (ATCC CCL61); 293HEK human kidney epithelial cells (ATCC CRL-11268); primary mouse embryonic fibroblasts from TLR2, TLR4, or MyD88 knockout mice; Human Umbilical Vein Endothelial Cells (HUVEC); human colonic carcinoma T84 cells (ATCC CCL248); END1 cervix epithelial cells; and monocyticlike JOSK-M cells (DSM ACC30).

Techniques: Transfection, Incubation, Infection, Bacteria, Staining, Real-time Polymerase Chain Reaction, Expressing